Estimation of Azadirachtin Content in Neem Extracts and Formulations

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Abstract
A high performance liquid chromatographic reversed-phase procedure was developed whereby azadirachtin content can be estimated in crude extracts of neem [Azadirachta indica] and in dust formulations of neem. An estimation of the azadirachtin content is achieved through the use of an external azadirachtin standard and valley-to-valley integration. Since azadirachtin seems to be the most potent insect feeding deterrent in these extracts and formulations, its content is a measurement of potency and represents an attempt at standardization.