Amino-Acid Sequence of the β1 Isosubunit of Taipoxin, an Extremely Potent Presynaptic Neurotoxin from the Australian Snake Taipan (Oxyuranus s. scutellatus)
- 3 March 2005
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 128 (1) , 71-75
- https://doi.org/10.1111/j.1432-1033.1982.tb06933.x
Abstract
The amino acid sequence of the β1 isosubunit of taipoxin, an extremely potent presynaptic neurotoxin from the Australian snake taipan, has been determined. The β1 isosubunit, which is neither toxic nor enzymatically active on its own, consists of a single polypeptide chain of 118 amino acids. The main fragmentation of the reduced and S‐carboxymethylated derivative was accomplished by cleavage with Staphylococcus aureus V8 protease. Tryptic peptides were used to align and complete the sequence, which was determined by automated Edman degradation. The taipoxin β1 isosubunit is closely homologous to the taipoxin α and γ subunits and to enzymatically active pancreatic and elapid snake venom phospholipases A2.This publication has 16 references indexed in Scilit:
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