CONCENTRATION OF OFLOXACIN IN CANINE PROSTATE TISSUE AND PROSTATE FLUID AFTER INTRAPROSTATIC INJECTION OF BIODEGRADABLE SUSTAINED-RELEASING MICROSPHERES CONTAINING OFLOXACIN

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Abstract
Excellent treatment results in chronic prostatitis by direct intra-prostatic injection of antibiotic were reported several decades ago with only minimal scientific background. We examined the distribution, in prostatic tissue and fluid, of the antibiotic in canines after intra-prostatic injection of biodegradable sustained-releasing microspheres containing 12 mg. of ofloxacin. A total of 36 male dogs, 12 controls and 24 experimental, older than 2 years, were used. Experimental dogs were given biodegradable sustained releasing microspheres containing ofloxacin 12 mg. and poly(D, L-lactic) acid 28 mg., designed to release over more than a 4 week period. The 12 control animals were divided into 2 groups, and oral ofloxacin 100 mg. was given twice a day for 2 and 4 weeks. The 24 experimental animals were divided into 4 subgroups of 6 dogs each, 4 for prostatic tissue and 2 for prostatic fluid level of ofloxacin determination. Anesthesia was initiated with ketamine HCl and xylazine, and maintained with intermittent ketamine HCl. In the experimental groups, 1 ml. of resolved formula was injected into one lobe of surgically exposed prostates. The concentration of ofloxacin was measured by high performance liquid chromatography (HPLC) of blood, prostatic tissue and prostatic fluid. Pilocarpine 0.5 mg./kg. was used for the collection of the prostatic fluid. The total ofloxacin of controls were 2,800 (2 weeks) and 5,600 (4 weeks) mg. In control groups, tissue concentrations of ofloxacin were relatively even at all segments of prostate, 7.4 ± 0.8 (2 weeks) and 9.2 ± 1.1 μg./ml. (4 weeks). The blood level ranged between 3.6 to 5.1 μg./ml. The prostatic fluid level ranged from 3.1 to 5.7 μg./ml. In the experimental groups, the tissue levels of ofloxacin were 10.5 ± 3.0 (1 week), 13.8 ± 4.5 (2 weeks), 7.1 ± 0.9 (3 weeks) and 7.7 ± 3.0 μg./ml. (4 weeks) in the injected lobe. The opposite lobes were 8.0 ± 1.1 (1 week), 10.2 ± 4.2 (2 weeks), 5.1 ± 1.4 (3 weeks) and 7.6 ± 0.8 (4 weeks) μg./ml. The blood level in the experimental groups ranged between 0.16 to 0.59 μg./ml. The prostate fluid level ranged from 2.9 to 6.1 μg./ml. in 8 dogs. Upon pathologic examination, the microspheres were interposed between prostate stroma and their size was reduced over time. Our study indicates that there is communication between the right and left prostate lobes. Direct injection of biodegradable sustained releasing ofloxacin formula into the prostate may be a substitute for long term antibiotic medication in humans for chronic prostatitis in the future without hurting the minimal inhibitory concentration(MIC)90.