The Effects of Bacteria on the Growth of Trichomonas foetus (Protozoa)

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Abstract
Certain bacteria accelerated and others inhibited the growth of T. foetus in vitro as detd. by direct microscopic counts of the tri-chomonads with a hemocytometer. Bacteria which inhibited growth were Corynebacterium diphtheriae, C. pyogenes, C. xerosis, Staphylococcus albus, S. aureus, Streptococcus bovis (5 strains), S.faecalis (2 strains), S. salivarius, S. hemolyti-cus, Shigella dysenteriae, S. paradysenteriae, Escherichia coli and Aerobacter aerogenes, as well as Vibrio comma, Neisseria catarrhalis, Strep. pyogenes, C. hofmannii and C. renale. Bacteria that had no pronounced effect on the growth of T. foetus or which slightly accelerated multiplication were Bacillus subtilis, Pseudomonas aeruginosa, Sarcina sp. and 9 cultures of diphtheroids isolated from the genital tract of clinically normal breeding bulls. Brucella abortus (strain 19) induced rapid multiplication of the trichomonads but did not permit as long a survival time as the control cultures. C. equi prolonged the life of T. foetus some 288 hours beyond the 240-hr. limit of pure cultures of this trichomonad. Bacterial filtrates and heat-killed cultures which were tested affected trichomonad growth in a manner comparable to the corresponding living bacteria cultured with T. foetus.

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