Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations
Open Access
- 1 September 1995
- journal article
- Published by Springer Nature in British Journal of Cancer
- Vol. 72 (3) , 696-701
- https://doi.org/10.1038/bjc.1995.396
Abstract
We examined DNA from 116 female and four male breast cancer patients for loss of heterozygosity (LOH). DNA was analysed by polymerase chain reaction using ten microsatellite markers on chromosome 11. Three distinct regions of LOH were identified: 11p15.5, 11q13 and 11q22-qter with a LOH frequency of 19, 23 and 37-43% respectively. The marker D11S969 showing the highest frequency of LOH (43%) is located at the 11q24.1-q25 region. No previous molecular genetic studies have shown frequent LOH at the region telomeric to q23 on chromosome 11. Southern analysis revealed that LOH at 11q13 was due to amplification, whereas LOH at 11q22qter was due to deletion. LOH at 11p15.5 was associated with paucity of hormone receptor proteins, high S-phase and positive node status. An association was found between LOH at 11q13 and positive node status. LOH at the 11q22-qter region correlated with a high S-phase fraction. A significant association was found between LOH at 11p15 and chromosome regions 17q21 (the BRCA1 region) and 3p.Keywords
This publication has 32 references indexed in Scilit:
- The 1993–94 Généthon human genetic linkage mapNature Genetics, 1994
- Relationship between loss of estrogen and progesterone receptor expression and of 6q and 11q chromosome arms in breast cancerInternational Journal of Cancer, 1994
- Absence of linkage to the ataxia telangiectasia locus in familial breast cancerHuman Genetics, 1993
- Incidence of Cancer in 161 Families Affected by Ataxia–TelangiectasiaNew England Journal of Medicine, 1991
- Preferential involvement of 11q23–24 and 11p15 in breast cancerCancer Genetics and Cytogenetics, 1991
- Dinucleotide repeat polymorphism at the D11S35 locusNucleic Acids Research, 1990
- Localization of an ataxia-telangiectasia gene to chromosome 11q22–23Nature, 1988
- ALLELE LOSS ON SHORT ARM OF CHROMOSOME 17 IN BREAST CANCERSThe Lancet, 1988
- Multiple endocrine neoplasia type 1 gene maps to chromosome 11 and is lost in insulinomaNature, 1988
- AMPLIFICATION OF c-erbB-2 ONCOGENE IN HUMAN ADENOCARCINOMAS IN VIVOThe Lancet, 1986