Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma

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Abstract
Papilloma virus DNA from a laryngeal papilloma was cloned in phage .lambda. L47 and characterized after cleavage with different restriction enzymes. Hybridization with the DNA of human papilloma virus types 1, 2, 3, 4, 5 and 8 showed no homology under stringent hybridization conditions. Human papilloma virus type 6 DNA was partially identical to laryngeal papilloma virus DNA; different restriction enzyme fragments hybridizing with the other DNA were identified on each genome. The degree of homology was determined by reassociation kinetics to be 25%. According to the present nomenclature, laryngeal papilloma virus therefore represents a different type of human papilloma virus and is tentatively designated as human papilloma virus type 11. Sequences homologous to laryngeal papilloma virus DNA were also found in 4 of 9 additional laryngeal papillomas. Attempts to detect homologous DNA in 12 carcinomas of the larynx were negative.