A New Analytical Method For Determining Duration of Phases, Rate of Dna Synthesis and Degree of Synchronization From Flow-Cytometric Data On Synchronized Cell Populations

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Abstract
L-cells [mouse fibroblast] synchronized by mitotic selection were investigated by flow-cytometry and the fractions of cells in the various cell cycle compartments were determined as a function of time. A new analytical evaluation procedure was developed, by which the mean transit-times of cells through various cell cycle phases can be calculated from these data. The examples for application of the method presented are determination of the duration of G1, S, G2 + M and of the whole cell cycle, calculation of the rate of DNA synthesis in several subcompartments of the S-phase and evaluation of the degree of synchronization at different stages of the cell cycle.