Determination of the length distribution of poly(A) at the 3′ terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA
- 1 January 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 7 (5) , 1195-1204
- https://doi.org/10.1093/nar/7.5.1195
Abstract
Rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(A) in an RNA population can be obtained by 3'-terminal 32P-labelling of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3'-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented.Keywords
This publication has 17 references indexed in Scilit:
- Cell-free synthesis and processing of the proteins of poliovirus.Proceedings of the National Academy of Sciences, 1978
- 3′-Terminal labelling of RNA with T4 RNA ligaseNature, 1978
- Isolation and characterization of polyadenylate-containing RNA from Bacillus brevisBiochemistry, 1978
- The use of thin acrylamide gels for DNA sequencingFEBS Letters, 1978
- The 3′‐Terminal Nucleotide Sequence of Encephalomyocarditis Virus RNAEuropean Journal of Biochemistry, 1978
- Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized filmFEBS Letters, 1977
- The dynamics of maternal poly(A)-containing mRNA in fertilized sea urchin eggsCell, 1977
- Cowpea mosaic virus RNAs have neither m7GpppN … nor mono-, di- or triphosphates at their 5′ endsCell, 1977
- The Size and Location of the Poly(A) Tract in EMC Virus RNAJournal of General Virology, 1977
- Size of the Poly(A) Sequences in Encephalomyocarditis Virus RNAIntervirology, 1975