ASSIGNMENT OF THE GENE FOR ACID BETA-GLUCOSIDASE TO HUMAN CHROMOSOME-1

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 33  (4) , 564-575
Abstract
The structural gene for human acid .beta.-glucosidase (GBA) was assigned to chromosome 1 using somatic cell hybridization techniques for gene mapping. The human enzyme was detected in mouse RAG cell-human fibroblast cell hybrids by a sensitive double antibody immunoprecipitation assay using a mouse antihuman GBA antibody. No cross-reactivity between mouse .beta.-glucosidase and human GBA or neutral .beta.-glucosidase (GBN) was observed. Primary, secondary and tertiary man-mouse hybrid lines [52], derived from 3 separate fusion experiments, were analyzed for human GBA and enzyme markers for the human chromosomes. Human GBA in these hybrid clones was correlated with the presence of human chromosome 1 or its enzymatic markers, phosphoglucomutase 1 (PGM1) and fumarate hydratase (FH). All other human chromosomes were eliminated by the independent segregation of GBA and their respective enzyme markers and/or chromosomes. Using a RAG .times. human fibroblast line with a mouse-human rearrangement of human chromosome 1, the locus for GBA was limited to the region 1p 11 to 1qter.