THE SEROLOGICAL ACTIVITY OF DENATURED ANTIBODIES

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Abstract
A difference was found in combining ratio between native, irreversibly denatured, and regenerated antibodies obtained from Type I antipneumococcus horse serum. This may be ascribed either to a change in effective antibody valence or to changes in their molecular dimensions. The regenerated antibody is nearly as fully precipitable by the homologous antigen (70%) as is the native (80%), and the irreversibly denatured material is even more so (50-60% as against 37%). This suggests that, contrary to Pauling''s hypothesis, the difference between antibody globulin and normal globulin is not merely one of steric arrangement, but probably one of amino-acid composition.

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