MECHANISM OF CORNEAL WOUND HEALING
- 1 August 1949
- journal article
- research article
- Published by American Medical Association (AMA) in Archives of Ophthalmology (1950)
- Vol. 42 (2) , 140-147
- https://doi.org/10.1001/archopht.1949.00900050145004
Abstract
IN SPITE of the large amount of clinical and laboratory research work1that has been done, very little is actually known about the mechanism of corneal wound healing and the cells involved in this healing process. To this end we have aimed our research: first, to identify the cells involved and, second, to learn the mechanism of corneal wound healing. METHOD A method has been worked out for maintaining rabbit corneal tissue in vitro apparently for an indefinite period. Fresh rabbit cornea is removed from the eye of the rabbit. The cornea is cut into pieces approximately 2 mm. square and 0.5 mm. thick. These pieces of tissue are planted in 3.5 D. Carrel flasks containing as a basic medium 0.7 cc. of Tyrode's solution, 0.3 cc. of rabbit serum, 0.5 cc. of chicken plasma and 1 drop of tissue extract of chick embryonic tissue (to facilitate formation ofKeywords
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