MONOCLONAL-ANTIBODY AGAINST A MEMBRANE ANTIGEN CHARACTERIZING LEUKEMIC HUMAN LYMPHOCYTE-B

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (5) , 1927-1934
Abstract
In the diagnosis of non-Hodgkins''s lymphomas, the ready characterization of the neoplastic cell lineage by analysis of cell surface markers is of great importance. Evidence for the existence of a human B-leukemia-associated antigen recognized by a complement-fixing monoclonal antibody (anti-Y 29/55) is presented. A hybridoma was produced by fusing mouse myeloma cells and splenocytes of a mouse immunized against lymphoid cells of a patient with B-cell chronic lymphocytic leukemia. Characterization of anti-Y 29/55-reactive normal and malignant leukocytes was demonstrated by cytolysis and indirect immunofluorescence. This revealed reactivity with an antigen on B-lymphoma cells (11 patients), on leukemic lymphocytes in B-cell chronic lymphocytic leukemia (13 patients) and on malignant cells in hairy-cell leukemia (2 patients) but not on leukemic cells of T-cell acute lymphoblastic leukemia (1 patient), on T-lymphoma cells (1 patient), on cells of acute myeloblastic leukemia (4 patients), or of chronic myeloid leukemia (4 patients). No specific cytolysis occurred with B- and T-peripheral blood lymphocytes from healthy donors (16 individuals), patient with reactive lymphocytosis (1 patient), nonleukemic multiple myeloma (6 patients), or Hodgkin''s disease (3 patients). Surface Ig-positive, sheep RBC-negative lymphocytes from human spleen (3 individuals), tonsils (7 individuals) and lymph nodes (1 individual) were recognized. Leukemic B-cells apparently carry a marker characteristic of nonrecirculating sessile B-lymphocytes.