Wound-Induced Resistance to Cellulase in Oat Leaves

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Abstract
Peeling the epidermis induces the development of resistance to cellulolytic digestion in the mesophyll cell walls of the first leaf of 1- to 3-wk-old oat seedlings (A. sativa var. Victory). Development of resistance occurs between 3 and 11 h after the abaxial epidermis is peeled from the blade and is inhibited by actinomycin D (20 .mu.g/ml) or cycloheximide (1 .mu.g/ml). Other methods of wounding (cutting with a razor blade, stabbing with a dissection needle or brushing with diatomaceous silica) also induce resistance in cells near the wounds. Peeling similarly induces resistance to the digestion of mesophyll cell walls by cellulysin (Calbiochem) in pea, corn, wheat and barley. [Pisum sativum cv. Alaska, Zea mays, Triticum aestivum cv. Yamhill and Hordeum vulgare cv. Himalaya.].