Trans-acting regulatory variation in Saccharomyces cerevisiae and the role of transcription factors
Top Cited Papers
- 3 August 2003
- journal article
- Published by Springer Nature in Nature Genetics
- Vol. 35 (1) , 57-64
- https://doi.org/10.1038/ng1222
Abstract
Natural genetic variation can cause significant differences in gene expression, but little is known about the polymorphisms that affect gene regulation. We analyzed regulatory variation in a cross between laboratory and wild strains of Saccharomyces cerevisiae. Clustering and linkage analysis defined groups of coregulated genes and the loci involved in their regulation. Most expression differences mapped to trans-acting loci. Positional cloning and functional assays showed that polymorphisms in GPA1 and AMN1 affect expression of genes involved in pheromone response and daughter cell separation, respectively. We also asked whether particular classes of genes were more likely to contain trans-regulatory polymorphisms. Notably, transcription factors showed no enrichment, and trans-regulatory variation seems to be broadly dispersed across classes of genes with different molecular functions.Keywords
This publication has 39 references indexed in Scilit:
- Sequencing and comparison of yeast species to identify genes and regulatory elementsNature, 2003
- In vivo characterization of regulatory polymorphisms by allele-specific quantification of RNA polymerase loadingNature Genetics, 2003
- Natural variation in human gene expression assessed in lymphoblastoid cellsNature Genetics, 2003
- Transcriptional Regulatory Networks in Saccharomyces cerevisiaeScience, 2002
- Detection of regulatory variation in mouse genesNature Genetics, 2002
- Variation in gene expression within and among natural populationsNature Genetics, 2002
- Functional profiling of the Saccharomyces cerevisiae genomeNature, 2002
- Yeast Cbk1 and Mob2 Activate Daughter-Specific Genetic Programs to Induce Asymmetric Cell FatesCell, 2001
- The Transcriptional Program of Sporulation in Budding YeastScience, 1998
- Gene disruption with PCR products in Saccharomyces cerevisiaeGene, 1995