Mutants That Produce Nitrogenase in the Presence of Ammonia
Open Access
- 1 December 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (12) , 3501-3503
- https://doi.org/10.1073/pnas.69.12.3501
Abstract
Mutants of Azotobacter vinelandii that fix N(2) in the presence of excess NH(4) (+) have been isolated. A mutant that was unable to synthesize component I and component II of nitrogenase was spontaneously reverted to the N(2)-fixing phenotype. Of 21 revertants picked, 7 revertants were not as sensitive as the wild type to repression. A derepressed mutant is as sensitive as the wild type to growth inhibition by 2-methylalanine in the presence of glucose.Keywords
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