THE INFLUENCE OF INHIBITORS OF CELLULAR RESPIRATION ON THE PRODUCTION OF STRUCTURAL MUTATIONS IN HUMAN-LYMPHOCYTES IRRADIATED DURING DIFFERENT STAGES OF THE MITOTIC-CYCLE

  • 1 January 1985
    • journal article
    • research article
    • Vol. 21  (2) , 252-261
Abstract
Human lymphocytes were irradiated by 60Co .gamma.-rays after 0, 10, 20, 35, 45, 48 and 49.5 h of incubation. Immediately after irradiation sodium cyanide, sodium fluoride or monoiodoacetic acid was given for 2.5 h. Non-irradiated cells were subjected to the same treatments. Chromosomal aberrations were analyzed in metaphase cells of the first mitosis. When administered alone, all chemicals increased the frequency of chromatid aberrations. The special analysis showed that these chemicals were not mutagens in a strict sense, as the observed increase of aberration frequency was due to inhibition of repair processes, which increased the probability of manifestation of spontaneous changes (so-called pseudomutagenesis). The same chemicals increased the frequency of radiation-induced aberrations during 2 periods of the mitotic cycle, namely, in the end of the G1 stage and in the G2 stage. It has been recently shown that the inhibitor of DNA synthesis, 5-fluorodeoxyuridine, increased the frequency of radiation-induced aberrations during the same periods. The process of repair proceeding during these periods requires both DNA synthesis and energy supply.

This publication has 0 references indexed in Scilit: