Methods for Sequencing Oligoribonucleotides and RNA by High Performance Liquid Chromatography1
- 1 October 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 86 (4) , 1081-1088
- https://doi.org/10.1093/oxfordjournals.jbchem.a132602
Abstract
Conditions were established for the separation and quantitative determination of ribonucleo-sides, mono- and oligo-ribonucleotides by high-performance liquid chromatography (HPLC) on columns of AS-Pellionex SAX and AL-Pellionex WAX. By combining a high-speed UV spectrum monitor with an HPLC apparatus, products of RNase digestions of oligo-nucleotides and 5S ribosomal RNA (rRNA) were identified by measuring their UV spectra under continuous solvent flow, and also from their retention times on the columns (positions of elution). It took only 10 to 30 min for one chromatography run and required less than 0.01 A260 unit of sample per nucleotide material in each peak.Keywords
This publication has 1 reference indexed in Scilit:
- Nucleotide Sequence of 5S Ribosomal RNA from Rainbow Trout (Salmo gairdnerii) Liver1The Journal of Biochemistry, 1979