Statistical exploration of variation in quantitative two‐dimensional gel electrophoresis data

2 December 2004

journal article
research article
Published by Wiley in Proteomics

Vol. 4 (12) , 3791-3799
https://doi.org/10.1002/pmic.200300824

Abstract

Two‐dimensional gel electrophoresis is a major technique in global analysis at the protein level. This paper presents an examination of spot volume data from three gel sets with radioactively labeled yeastSaccharomyces cerevisiaeproteins. A strong varianceversusmean dependence in data was found to be stabilized by applying a shifted logarithmic transformation. However, transformed data showed a remaining substantial variance heterogeneity for different proteins. Furthermore, examination of studentized residuals revealed that transformed data were approximately normally distributed and that there were spatial correlations among the measurement errors in the gel.

Keywords

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