A simple, nondestructive spraying assay for the detection of an active kanamycin resistance gene in transgenic tomato plants
- 1 August 1989
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 78 (2) , 169-172
- https://doi.org/10.1007/bf00288794
Abstract
A simple, nondestructive kanamycin spraying assay for detecting neomycin phosphotransferase II activity in tomato has been developed. This assay does not require the use of tissue culture or biochemical methods, but allows transgenic and non-transgenic tomato plants to be distinguished directly at the plant level in the green-house. Its potential applications in large-scale genetic analyses are discussed.This publication has 14 references indexed in Scilit:
- A simplified method for the detection of neomycin phosphotransferase II activity in transformed plant tissuesPlant Molecular Biology Reporter, 1987
- Locations and stability of Agrobacterium-mediated T-DNA insertions in the Lycopersicon genomeMolecular Genetics and Genomics, 1986
- Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciensPlant Cell Reports, 1986
- Genetic transformation in higher plantsCritical Reviews in Plant Sciences, 1986
- Breeding of a tomato genotype readily accessible to genetic manipulationPlant Science, 1986
- Simple binary vectors for DNA transfer to plant cellsPlant Molecular Biology, 1985
- A Simple and General Method for Transferring Genes into PlantsScience, 1985
- A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extractsGene, 1984
- Inheritance of Functional Foreign Genes in PlantsScience, 1984
- A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 1983