CONSTRUCTION OF FLOCCULENT BREWER'S YEAST BY CHROMOSOMAL INTEGRATION OF THE YEAST FLOCCULATION GENEFLO1

Open Access

4 March 1994

journal article
research article
Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing

Vol. 100 (2) , 73-77
https://doi.org/10.1002/j.2050-0416.1994.tb00809.x

Abstract

Yeast flocculation gene FLO1, located on chromosome I of Saccharomyces cerevisiae, has been cloned previously¹⁶. However, it has recently been found that the gene was an in-frame deletion derivative of the chromosomal intact FLO1 gene¹⁹. When introduced into non-flocculent industrial strains, including brewer's yeast, the latter gene, FLO1L, containing an open reading frame of 4,611 bp, conferred stronger flocculation than the former gene, FLO1S, containing an open reading frame of 2,586. By chromosomal integration of the ADH1-controlled FLO1L gene, “gene therapy” of the flocculation behaviour of the parent non-flocculent brewer's yeast was successfully achieved.

Keywords

This publication has 3 references indexed in Scilit:

Breeding of flocculent brewer’s yeast by genetic engineering
Published by Oxford University Press (OUP) ,1991
Genetic Control of Flocculation
Published by Springer Nature ,1983
Malting and Brewing Science
Published by Springer Nature ,1982