DNA sequence of the araC regulatory gene from Escherichia coli B/r
Open Access
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (22) , 5267-5274
- https://doi.org/10.1093/nar/8.22.5267
Abstract
The DNA sequence of the araC regulatory gene from Escherichia coli B/r has been determined by the base-specific chemical cleavage reactions of Maxam and Gilbert. An open reading frame is found which codes for a protein of 292 amino acids. A nonsense mutation, araC5, is shown to result from a G to A transition at nucleotide 429 converting the tryptophan codon TGG to the amber codon TAG. A deletion which does not recombine with any known point mutation In araC, Δ(araC0)719, removes all but the last 22 codons of the gene.Keywords
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