Bacterial Na+‐ATP synthase has an undecameric rotor
Open Access
- 1 March 2001
- journal article
- research article
- Published by Springer Nature in EMBO Reports
- Vol. 2 (3) , 229-233
- https://doi.org/10.1093/embo-reports/kve047
Abstract
Synthesis of adenosine triphosphate (ATP) by the F1F ATP synthase involves a membrane‐embedded rotary engine, the F domain, which drives the extra‐membranous catalytic F1 domain. The F domain consists of subunits a 1 b 2 and a cylindrical rotor assembled from 9–14 α‐helical hairpin‐shaped c ‐subunits. According to structural analyses, rotors contain 10 c ‐subunits in yeast and 14 in chloroplast ATP synthases. We determined the rotor stoichiometry of Ilyobacter tartaricus ATP synthase by atomic force microscopy and cryo‐electron microscopy, and show the cylindrical sodium‐driven rotor to comprise 11 c ‐subunits.Keywords
This publication has 22 references indexed in Scilit:
- Molecular Architecture of the Rotary Motor in ATP SynthaseScience, 1999
- Electrostatically Balanced Subnanometer Imaging of Biological Specimens by Atomic Force MicroscopeBiophysical Journal, 1999
- Energy and entropy balance of ATP synthesisBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1997
- ATP synthase: an electrochemical ransducer with rotatory mechanicsTrends in Biochemical Sciences, 1997
- Adsorption of Biological Molecules to a Solid Support for Scanning Probe MicroscopyJournal of Structural Biology, 1997
- The H+/ATP coupling ratio of the ATP synthase from thiol‐modulated chloroplasts and two cyanobacterial strains is fourFEBS Letters, 1996
- Structure at 2.8 Â resolution of F1-ATPase from bovine heart mitochondriaNature, 1994
- Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopyJournal of Molecular Biology, 1990
- Cryo-electron microscopy of vitrified specimensQuarterly Reviews of Biophysics, 1988
- Structure of purple membrane from halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolutionUltramicroscopy, 1986