Amino acid incorporation by preparations from the developing rat brain
- 1 May 1966
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 99 (2) , 261-265
- https://doi.org/10.1042/bj0990261
Abstract
A study has been made of the ability of cerebral microsome-cell sap systems, taken from rats at various ages, to incorporate [C14]-valine. The systems appear to follow Michaelis-Menten kinetics when cell sap is considered as substrate, microsomes as enzyme and total counts/mg. of microsomal protein as a measure of reaction velocity. Reproducible "affinity constant" values are obtained, and the system from 4-day-old rats has a higher Vmax. and affinity constant than the system from adult rats. It is suggested the amino acid-incorporating ability of different systems may be compared by this means.This publication has 13 references indexed in Scilit:
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