USE OF AN ION-EXCHANGER AS A RAPID MEANS OF EXTRACTION APPLIED TO ESTIMATION OF ESTROGEN AND URINARY PREGNANDIOL DURING PREGNANCY BY COLORIMETRY OR BY GAS-CHROMATOGRAPHY

  • 1 January 1976
    • journal article
    • research article
    • Vol. 34  (6) , 423-430
Abstract
The method described permits, after classical hydrolysis, extraction without a solvent of estrogens during pregnancy. It also permits estimation of pregnanediol which is eluted in a different fraction. Twenty estimates may be carried out in 1/2 a day on test samples of from 2-10 ml of urine. The estimation of total estrogen by colorimetry takes into consideration the efficiency of extraction by the use of a standard range treated under the same conditions. For gas chromatography, 2 internal standards are added to the urine after hydrolysis: these are 16,17-epiestriol and 5 .beta. pregnane-3 .alpha., 17 .alpha., 20 .beta. triol (PGTS); they have, respectively, the same behavior as estriol on the one hand and pregnanediol and pregnanetriol, on the other hand. They take into consideration resin extraction and chromatographic behavior and may be used for quantitative estimation. The graphs of standardization and recovery were studied for the main estrogens together with pregnanediol. The various eluates were controlled by gas chromatography coupled to mass spectrometry.