Isolation of human intestinal defensins from ileal neobladder urine

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Abstract
We describe the isolation of naturally occurring human intestinal defensins HD‐5 and HD‐6 from ileal neobladder urine and ileal mucosa. Using an antibody‐based detection assay, we found multiple N‐terminally processed forms of HD‐5. The predominant HD‐5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23–94 and 29–94 versus aa 36–94, 56–94 and 63–94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD‐6 yielded aa 69–100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion.