ETHER PHOSPHOLIPIDS IN GUINEA-PIG POLYMORPHONUCLEAR LEUKOCYTES AND MACROPHAGES - OCCURRENCE OF HIGH-LEVELS OF 1-ORTHO-ALKYL-2-ACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 712  (3) , 515-522
Abstract
Significant proportions of the choline phosphoglycerides (CPG) were found to contain alkyl ether-type moieties (e.g., 1-O-alkyl-2-acyl-glycero-3-phosphocholine) in both guinea pig peritoneal exudate polymorphonuclear leukocytes (16.4%) and macrophages (13.5). High proportions of the ethanolamine phosphoglycerides (EPG) contained alkenyl ether moieties in both cells (37.2 and 41.2%), while the proportions of the CPG containing alkenyl moieties and of the EPG containing alkyl moieties were small. The ether phospholipid composition as well as the fatty chain profiles of these 2 types of cells had relatively similar patterns. The fatty chains at the 1- and 2-positions for alkenyl ether, alkyl ether and diacyl phosphoglycerides showed considerable differences. The amount of 16:0 at the 1-position was higher in alkyl compounds than that in diacyl compounds of the CPG. This was also the case in ether-containing and diacyl EPG. The most predominant fatty acids at the 2-position was 18:2, in each lipid class, except for the alkenyl CPG. The amounts of 20:4 and other polyunsaturated fatty acids were low in every lipid class, though ether compounds contained higher amounts of 20:4 than diacyl compounds, particularly for EPG.