Transformation of the cyanobacterium Anacystis nidulans 6301 with the Escherichia coli plasmid pBR322.
- 1 April 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (8) , 2546-2550
- https://doi.org/10.1073/pnas.83.8.2546
Abstract
A. nidulans 6301 has been transformed in the light to ampicillin resistance with the plasmid pBR322. Permeaplasts prepared by 2-hr treatment of cells with lysozyme and EDTA are transformed with a 50-fold higher efficiency than that observed for cells. .beta.-Lactamase is present in A. nidulans transformed either with pBR322 or the plasmid pCH1 as evidenced by hydrolysis of the .beta.-lactam ring of Nitrocefin in extracts of transformants. .beta.-Lactamase also can be immunoprecipitated from extracts of [35S]methionine-labeled pBR322 transformants and coprecipitates with ribulose-biphosphate carboxylase. Expression of the carboxylase is apparently amplified in pBR322 transformants as is that for several soluble proteins in pCH1 transformants. Chromosomal DNA per cell is increased about 6-fold after transformation of A. nidulans 6301 with either pBR322 or pCH1. A 4.3-kilobase-pair plasmid can be isolated from pBR322 transformants in addition to the endogenous plasmids pUH24 and pUH25.This publication has 31 references indexed in Scilit:
- Radioisotopic evidence for the polypeptides associated with photosystem IIBiochemical and Biophysical Research Communications, 1984
- Length of foreign DNA in chimeric plasmids determines the efficiency of its integration into the chromosome of the cyanobacterium Synechococcus R2Gene, 1984
- Stable integration of foreign DNA into the chromosome of the cyanobacterium Synechococcus R2Gene, 1983
- Amplification of a chimeric plasmid carrying an erythromycin-resistance determinant introduced into the genome of Streptococcus pneumoniaeGene, 1983
- A new approach for molecular cloning in cyanobacteria: cloning of an anacystis nidulans met gene using a Tn 907-induced mutantGene, 1982
- Vectors for cloning in cyanobacteria: Construction and characterization of two recombinant plasmids capable of transformation to Escherichia coli K12 and Anacystis nidulans R2Molecular Genetics and Genomics, 1981
- Cloning of chromosomal genes in Streptococcus pneumoniae.Proceedings of the National Academy of Sciences, 1981
- Photosynthetic Properties of Permaplasts of AnacystisPlant Physiology, 1972
- Novel Method for Detection of β-Lactamases by Using a Chromogenic Cephalosporin SubstrateAntimicrobial Agents and Chemotherapy, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970