Regulation of SV40‐induced cell division and tumor antigen by dibutyryl adenosine 3′‐5′‐monophosphate

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Abstract
Simian virus 40 (SV40) induces cell division in microcultures of sparsely plated nongrowing mouse BALB/3T3 cells during acute infection at moderate multiplicities of infection (MOI = 10–100). The infected cells are killed when a MOI of 1,000 is used. SV40 tumor (T) antigen is synthesized in the infected cells, but viral DNA, virion antigen, and progeny virions are not synthesized (abortive infection). The addition of exogenous dibutyryl adenosine 3′‐5′‐monophosphate (dbcAMP) at the time of infection stimulates the SV40‐induced cell division at all MOI and inhibits SV40‐induced cell death at high MOI. The percentage of T antigen‐positive cells, as monitored by immunofluorescence, is also increased by the addition of dbcAMP at the time of infection. This regulation of SV40‐induced cell division and T antigen formation by exogenous dbcAMP occurs within the first 6 hr after infection at 37° C and is dependent upon both the MOI and the concentration of added dbcAMP. The addition of dbcAMP to productively infected TC7 monkey cells has little effect on the SV40‐induced cell death or T antigen formation.