Simultaneous Detection and Differentiation between Porcine Circovirus and Porcine Parvovirus in Boar Semen by Multiplex Seminested Polymerase Chain Reaction
Open Access
- 1 January 2003
- journal article
- Published by Japanese Society of Veterinary Science in The Journal of Veterinary Medical Science
- Vol. 65 (6) , 741-744
- https://doi.org/10.1292/jvms.65.741
Abstract
A multiplex seminested polymerase chain reaction (PCR) was developed for the simultaneous detection and differentiation among porcine circovirus 1 (PCV1), PCV2, and porcine parvovirus (PPV) from boar semen. Primers of PCV1, PCV2 and PPV were specific and did not react with other viruses respectively. Twenty (20.4%) and 42 (42.9%) out of 98 whole semen samples were found to be positive for PCV and PPV using multiplex conventional and seminested PCR, respectively. When the separated fractions of PCV or PPV-contaminated semen were analyzed using multiplex seminested PCR, PCV and PPV DNA were found to be present mainly in the seminal fluid and nonsperm cell fractions. This multiplex seminested PCR assay was sensitive, rapid and a good alternative method for the detection and differentiation of these viruses in boar semen.Keywords
This publication has 29 references indexed in Scilit:
- Differentiation of porcine circovirus 1 and 2 in formalin-fixed, paraffin-wax-embedded tissues from pigs with postweaning multisystemic wasting syndrome by in-situ hybridisationResearch in Veterinary Science, 2001
- Distribution of Porcine Parvovirus in Porcine Circovirus 2-infected Pigs with Postweaning Multisystemic Wasting Syndrome as shown by In-situ HybridizationJournal of Comparative Pathology, 2000
- Reproduction of Lesions of Postweaning Multisystemic Wasting Syndrome by Infection of Conventional Pigs with Porcine Circovirus Type 2 Alone or in Combination with Porcine ParvovirusJournal of Comparative Pathology, 2000
- In-situ Hybridization for the Detection of Porcine Circovirus in Pigs with Postweaning Multisystemic Wasting SyndromeJournal of Comparative Pathology, 1999
- Experimental Reproduction of Severe Wasting Disease by Co-infection of Pigs with Porcine Circovirus and Porcine ParvovirusJournal of Comparative Pathology, 1999
- Development of a PCR-based method coupled with a microplate colorimetric assay for the detection of Porcine Parvovirus and application to diagnosis in piglet tissues and human plasmaMolecular and Cellular Probes, 1998
- Characterization of novel circovirus DNAs associated with wasting syndromes in pigs.Journal of General Virology, 1998
- Isolation of Porcine Circovirus-like Viruses from Pigs with a Wasting Disease in the USA and EuropeJournal of Veterinary Diagnostic Investigation, 1998
- Sequence of porcine circovirus DNA: affinities with plant circoviruses.Journal of General Virology, 1997
- Isolation of Bovine Herpesvirus 1 from Preputial Swabs and Semen of Bulls with BalanoposthitisJournal of Veterinary Diagnostic Investigation, 1992