Investigation of the Influence of Particle Size on the Productivity of Preparative HPLC Columns

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Abstract
Changes in band profile, resolution, production, and yield with increasing sample size were determined for 1″ i.d. × 12″ preparative columns packed with 10 or 40 urn silica particles of the same origin. The results for a pure compound and for a mixture are compared. When a pure compound is injected, the efficiency of both columns decreases with increasing sample size. Although the 10-μm particle column is notably more efficient than the 40-μm particle column, for very large samples the plate numbers of both columns eventually become similar. When a binary mixture of isomers is injected, however, the 10-μm particle column provides a much larger yield and production of pure isomers than expected. When the sample size of this mixture is increased, the first eluted peak broadens to a lesser extent than if it were the peak of a pure component. It is better resolved from the more retained isomer than could be predicted on the basis of relative