Modulatory effects of curcumin on the chromosomal damage induced by doxorubicin in Chinese hamster ovary cells
- 22 March 1999
- journal article
- Published by Wiley in Teratogenesis, Carcinogenesis, and Mutagenesis
Abstract
Curcumin, a natural phenolic compound, is gaining importance as a free radical scavenger. This study was undertaken to investigate the modulatory effects of curcumin on the chromosomal damage induced by the antitumoral doxorubicin (DXR), a known free radical generator, in Chinese hamster ovary cells in culture. Cells were treated with three concentrations of curcumin (2.5, 5, or 10 μg/ml) and then treated with DXR (1.0 μg/ml) during different phases of the cell cycle. The results show that curcumin induces chromosomal damage in CHO at the highest concentration when compared to the untreated control. Neither treatment with curcumin shows a reduction in the clastogenicity of DXR. Instead, a statistically significant increase in the frequency of chromosomal damage was observed when the middle and the highest concentrations of curcumin were associated with DXR during the G1/S, S, and S/G2 phases of the cell cycle. The results clearly indicate the potentiating effect of curcumin on DXR‐induced chromosomal damage. Teratogenesis Carcinog. Mutagen. 19:1–8, 1999.Keywords
This publication has 30 references indexed in Scilit:
- Phenolics: Prooxidants or Antioxidants?Nutrition Reviews, 2009
- Effect of Concentration on the Cytotoxic Mechanism of Doxorubicin—Apoptosis and Oxidative DNA DamageBiochemical and Biophysical Research Communications, 1997
- Curcumin, an antioxidant and anti-tumor promoter, induces apoptosis in human leukemia cellsBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1996
- Oxidative mechanisms in the toxicity of metal ionsFree Radical Biology & Medicine, 1995
- Diminution of singlet oxygen-induced DNA damage by curcmin and related antioxidantsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1994
- Effect of β-carotene on clastogenic effects of mitomycin C, methyl methanesulphonate and bleomycin in Chinese hamster ovary cellsMutagenesis, 1994
- Effect of ascorbic acid and curcumin on quercetin-induced nuclear DNA damage, lipid peroxidation and protein degradationCancer Letters, 1992
- Curcumins as inhibitors of nitrosation in vitroMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1988
- Nonmutagenicity of curcumin and its antimutagenic action versus chili and capsaicinNutrition and Cancer, 1986
- Classification and relationships of induced chromosomal structual changes.Journal of Medical Genetics, 1976