Rate of sister chromatid exchanges in Bloom syndrome fibroblasts reduced by co-cultivation with normal fibroblasts.
- 1 March 1980
- journal article
- Vol. 32 (2) , 150-7
Abstract
Six strains of Bloom syndrome (BlS) fibroblasts responded to co-cultivation with normal fibroblasts at a 1:2 ratio by a reduced rate of sister chromatid exchanges (SCE's) from a mean of 67.5 (range = 59--78) to 28.4 (range = 21--35). The response was dose-dependent in one strain tested at 1:2, 1:1, and 2:1 ratios. In addition, quadriradial exchange figures and other signs of increased chromosomal instability were not found in BlS cells following co-cultivation with control cells. Control cells did not respond to BlS cells and maintained a normal rate of SCEs. Culture medium conditioned for 48 hrs by normal fibroblasts could also reduce the rate of SCEs in BlS fibroblasts, but less than in co-cultivation. We suggest that the reduced rate of SCEs and the lack of chromosomal instability in BlS cells following co-cultivation represent a corrective effect that is related to the basic defect and not dependent on cell-to-cell contact.This publication has 16 references indexed in Scilit:
- Bloom's Syndrome. VII. Progress report for 1978Clinical Genetics, 2008
- Normalisation of sister chromatid exchange frequencies in Bloom's syndrome by euploid cell hybridisationNature, 1979
- Frequency of sister chromatid exchanges in Bloom syndrome fibroblasts reduced by cocultivation with normal cellsHuman Genetics, 1979
- Effect of cocultivation on sister chromatid exchange frequencies in Bloom's syndrome and normal fibroblast cellsNature, 1978
- Suppression of the frequencies of sister chromatid exchanges in Bloom's syndrome fibroblasts by co-cultivation with Chinese hamster cellsHuman Genetics, 1978
- Chromosome aberrations and unscheduled DNA synthesis in X- and UV-irradiated lymphocytes from a boy with Bloom’s syndrome and a man with xeroderma pigmentosumCytogenetic and Genome Research, 1977
- Bloom's syndrome. V. Surveillance for cancer in affected familiesClinical Genetics, 1977
- Ultraviolet light sensitivity and delayed DNA-chain maturation in Bloom's syndrome fibroblastsNature, 1977
- Chromatid exchanges in ataxia telangiectasia, Bloom syndrome, Werner syndrome, and xeroderrna pigmentosumAnnals of Human Genetics, 1976
- Cytogenetic investigations in a new case of Bloom's syndromeHuman Genetics, 1976