A SIMPLE METHOD FOR THE ISOLATION OF FLAGELLA FROM TREPONEMA REITER

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Abstract
Flagella from T. reiteri were purified from a sonicate of the bacteria by diethylaminoethylcellulose chromatography followed by gel filtration on Sepharose CL-2B. The yield (0.8 mg flagellar protein per 8 g wet weight of treponemes) was identical to that obtained by previously described more time consuming methods. Crossed immunoelectrophoresis of the chromatographically isolated flagella against rabbit anti-T. reiteri Ig, monospecific anti-T. reiteri flagellar antibodies or human syphilitic serum in the upper gel showed 2 parallel but closely apposed precipitates. No contaminating material was found by EM of suspensions of flagella isolated by the new method. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of dissociated flagella showed 3 major and 1 minor band. Thus, the new method is seemingly quantitatively and qualitatively equal to earlier described purification procedures, but simpler to use.

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