L-Ascorbic Acid Determination in Fruits and Medical Formulations by Ion Interaction Reagent Reverse Phase HPLC Technique
- 1 April 1990
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 13 (7) , 1419-1434
- https://doi.org/10.1080/01483919008049258
Abstract
The content of L-ascorbic acid has been evaluated in juices of fruits and in typical medical formulations. Grapefruits, orange, lemon and kiwi, as well as tablets of vitamin C and antipyretic medicine added with vitamin C have been analyzed. The ion interaction reagent reverse phase HPLC chromatographic technique has been employed, making use of octylaminium salicylate as interaction reagent and of a C-18 spherical 5 μ m column as stationary phase. Sensitivity levels of the order of 100 pmoles can be obtained. The dependence on time and temperature of the oxidation processes which L-ascorbic acid undergoes during its preservation has also been studied.This publication has 18 references indexed in Scilit:
- Applications of a simultaneous assay of ascorbic acid, dehydroascorbic acid and ascorbic sulphate in biological materialsThe Analyst, 1989
- Spectrophotometric determination of L-ascorbic acid in pharmaceutical preparationsAnalytica Chimica Acta, 1988
- Quantitative Estimation of Dehydroascorbic Acid and Ascorbic Acid by High-Performance Liquid Chromatography—Application to Human Milk, Plasma, and LeukocytesJournal of Liquid Chromatography, 1988
- Simultaneous analysis of ascorbic and dehydroascorbic acid by high-performance liquid chromatography with post column derivatization and UV absorbanceJournal of Agricultural and Food Chemistry, 1986
- Polarographic method for the determination of μg-quantities of tungsten(VI)Analytical and Bioanalytical Chemistry, 1986
- Determination of ascorbic acid in soft drinks and fruit juices. Part 2. Applicability of direct ultraviolet spectrophotometry with background correctionThe Analyst, 1985
- Determination of ascorbic acid in its mixtures with thiols by reversed- phase high-performance liquid chromatography with electrochemical detection using a DC-TAST polarographJournal of Chromatography A, 1984
- Simultaneous determination of ascorbic acid and dehydroascorbic acid by reversed-phase ion-pair high-performance liquid chromatography with pre-column derivatisationJournal of Chromatography A, 1982
- Rapid high-performance liquid chromatographic determination of ascorbic acid and combined ascorbic acid-dehydroascorbic acid in beveragesJournal of Agricultural and Food Chemistry, 1981
- Resolution of ascorbic, dehydroascorbic and diketogulonic acids by paire-ion reversed-phase chromatographyJournal of Chromatography A, 1981