Organization of four mouse lambda light chain immunoglobulin genes.

Publisher Website
Google Scholar
Abstract
Four .lambda. L chain constant region (C) genes from mouse embryo DNA were cloned. Each carries its own joining (J) segment approximately 1.3 kilobases to its 5'' side. The 4 C genes occur in 2 clusters, 5'' J3C3J1C13'' and 5'' J2C2J4C43'', with C4 being a new C.lambda. gene. V.lambda.1 is joined productively with C.lambda.3 in a .lambda.3-producing myeloma; V.lambda.1 and V.lambda.2 may be the only V.lambda. genes. Based on the analysis of the germ line and rearranged variable region (V) .lambda. genes in myelomas it is argued that the V.lambda.1 and V.lambda.2 genes are at the 5'' side of the C3C1 and C2C4 clusters, respectively. The 2 clusters apparently arose by duplication. The role of J-associated DNA sequences in regulation of expression of the .lambda. subtypes was examined.