In Vitro and In Vivo Validation of ligA and tarI as Essential Targets in Staphylococcus aureus
Open Access
- 1 December 2008
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 52 (12) , 4470-4474
- https://doi.org/10.1128/aac.00548-07
Abstract
A conditional expression system has been developed using the isopropyl-β- d -thiogalactopyranoside (IPTG)-inducible P spac promoter to validate essential genes of Staphylococcus aureus in vivo. The system has been applied to prove the essentiality of ligA and to evaluate the function of tarI , which was found to be essential in vitro but not in vivo.Keywords
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