METABOLISM OF RNA AND PYRIMIDINE NUCLEOTIDES IN THE UTERUS DURING THE EARLY DECIDUAL CELL REACTION

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Abstract
Changes in the metabolism of pyrimidine 5′-nucleotides and RNA in the mouse uterus during the oil-induced decidual cell reaction were investigated. During the first 20 h after the intraluminal injection of 10 μl sesame oil the amounts of HClO4-soluble nucleotides and uridine 5′-nucleotides in the treated horn increased by 55% and 115%, respectively. During the same interval RNA increased by 101%, while the increase in protein was much smaller, and the amount of DNA remained almost unchanged. Doses of [2-14C]uridine, [5-3H]cytidine and [5-3H]orotic acid were administered to mice 20 min before killing. After [2-14C]uridine injection the specific activity of the uridine 5′-nucleotide fraction 20 h after the oil stimulus had not changed, while the specific activity of RNA increased by 43% during the 20-h interval. Similar results were obtained when [5-3H]-cytidine was injected. In contrast, after an injection of [5-3H]orotic acid the specific activities of both the uridine 5′-nucleotide and RNA fractions decreased with increasing time after the intraluminal injection of oil. When approximate rates of RNA synthesis were calculated independently from the data for [2-14C]uridine and [5-3H]orotic acid incorporation in these two fractions, the results agreed well, and indicated that the rate of synthesis of RNA in the decidualizing uterus increased by 64% and 163% at 8 and 20 h respectively after the intraluminal administration of sesame oil.
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