Estimation of Bilirubin Using Acetamide
- 1 March 1964
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 10 (3) , 197-213
- https://doi.org/10.1093/clinchem/10.3.197
Abstract
A study of the effect of acetamide as an accelerator of the reaction between free bilirubin and diazotized sulfanilic acid (DSA) has led to its use in a method for serum bilirubin which is simple, sensitive, linear over a wide range, and which requires neither excessive dilution nor protein precipitation. The conditions necessary for greatest sensitivity and for linearity have been established with regard to the pH of coupling and photometry, the concentration of DSA, the minimizing of turbidities, and the effect of protein.Keywords
This publication has 3 references indexed in Scilit:
- Analytic Methods for Bilirubin in Blood PlasmaClinical Chemistry, 1961
- A Study on the Estimation of Low Concentrations of Serum BilirubinAmerican Journal of Clinical Pathology, 1959
- The excretion of bilirubin as a diglucuronide giving the direct van den Bergh reactionBiochemical Journal, 1957