Isolation of in Vitro Synthesized Covalently Closed Circular Double-Stranded DNA by Selective Denaturation and Filtration
- 1 December 1984
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 14 (5) , 485-497
- https://doi.org/10.1080/00327488408061782
Abstract
Based upon their resistance to irreversible denaturation, covalently closed circular (CCC) DNA (non-nicked, double-stranded circular molecules) can be purified by alkaline denaturation, neutralization, and filtration through a nitrocellulose membrane. This procedure offers a simple means of isolating in vitro synthesized CCC DNA molecules. The preparations of molecules obtained by this method consisted of 91-97% CCC DNA and contained no detectable inhibitors of biological activity or enzymatic digestion.Keywords
This publication has 27 references indexed in Scilit:
- [32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectorsPublished by Elsevier ,1983
- [2] New M13 vectors for cloningPublished by Elsevier ,1983
- Oligonucleotide-directed mutagenesis of gene IX of bacteriophage M13Nucleic Acids Research, 1982
- Construction of viable and lethal mutations in the origin of bacteriophage φX174 using synthetic oligodeoxyribonucleotidesJournal of Molecular Biology, 1981
- The Isometric Single-Stranded DNA PhagesPublished by Springer Nature ,1977
- The mechanism of replication of φX174 single-stranded DNA: III. An enzymic study of the structure of the replicative form II DNAJournal of Molecular Biology, 1971
- Characterization of multiple circular DNA forms of colicinogenic factor E1 from Proteus mirabilisBiochemistry, 1968
- Purification of episomal DNA with cellulose nitrate membrane filtersBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- Preparation of double-stranded DNA (replicative form) of bacteriophage ΦX174: A simplified methodBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961