Determination of Amino Acids in Serum by Gas Liquid Chromatography
- 1 January 1976
- journal article
- research article
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 96 (6) , 777-782
- https://doi.org/10.1248/yakushi1947.96.6_777
Abstract
Determination of 22 amino acids, including asparagine and glutamine, in serum by GLC of N-isobutyloxycarbonyl (N-isoBOC) methyl ester derivatives was carried out. Serum samples were deproteinized with picric acid and purified with a cation-exchange resin. The amino acids obtained were derived to their N-isoBOC methyl esters and analyzed by GLC. Recoveries of amino acids added to sera ranged 93.1-100.5%; an exception was tyrosine which gave 70.3%. The data obtained by the GLC method were in good agreement with those by the automated amino acid analyzer.This publication has 2 references indexed in Scilit:
- Gas-liquid chromatographic analysis of protein amino acids as N-isobutyloxycarbonylamino acid methyl estersJournal of Chromatography A, 1976
- [3] Gas-liquid chromatography (GLC) of amino acid derivativesPublished by Elsevier ,1972