Measurement of Serum Folate by75Se-Selenofolate Radioassay: Results of a Multi-institutional Clinical Trial

Abstract
Waxman, Samuel, Schreiber, Carol, Rose, Michael, Johnson, Ian, Sheppard, Robin, Sumbler, Kenneth, Keen, Anne, and Guilford, Hugh: Measurement of serum folate by 75-Se-selenofolate radioassay. Results of a multi-institutional clinical trial. Am J Clin Pathol 70: 359–363,1978. The authors report the multi-institutional evaluation of a radioassay for serum folate utilizing a gamma-labeled folate analog (75Se-pteroyl-L-methylselenocysteine). The assay is run as a one-step simultaneous addition at ambient temperature and is completed within 45 minutes. The assay data were reproducible within four study centers and correlated with both the Lactobacillus casei diagnostic groups and the clinical pictures.

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