Molecular cloning of rat type 2C (IA) protein phosphatase mRNA.
- 1 March 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (6) , 1796-1800
- https://doi.org/10.1073/pnas.86.6.1796
Abstract
A full-length cDNA encoding rat type 2C (IA) protein phosphatase was isolated from a kidney cDNA library. The cDNA was identified by screening the library with oligonucleotides based on a partial amino acid sequence determined from purified rat liver phosphatase. This clone is 2.35 kilobase pairs long and has a single extended translation reading frame that predicts a 382-amino acid protein of 42,416 daltons. The deduced amino acid sequence contains segments corresponding to three peptides from rat liver type 2C protein phosphatase and two peptides from rabbit skeletal muscle type 2C phosphatase. Rat kidney type 2C protein phosphatase is distantly related to yeast adenylate cyclase but is not related to the catalytic subunits of two other protein phosphatases (types 1 and 2A).Keywords
This publication has 30 references indexed in Scilit:
- A second catalytic subunit of type‐2A protein phosphatase from rabbit skeletal muscleFEBS Letters, 1987
- Isolation and sequence analysis of a cDNA clone encoding a type‐1 protein phosphatase catalytic subunit: Homology with protein phosphatase 2AFEBS Letters, 1987
- Molecular cloning of cDNAs encoding two isoforms of the catalytic subunit of protein phosphatase 2ABiochemistry, 1987
- Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type‐2A protein phosphataseFEBS Letters, 1987
- Primary structure analysis proves that protein phosphatases 2C1 and 2C2 are isozymesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1987
- Localization of preangiotensinogen messenger RNA sequences in the rat brain.Hypertension, 1986
- Synthetic oligonucleotide probes deduced from amino acid sequence dataJournal of Molecular Biology, 1985
- Purification and Subunit Structure of Rat‐Liver Phosphoprotein Phosphatase whose Molecular Weight is 260000 by Gel Filtration (Phosphatase IB)European Journal of Biochemistry, 1980
- Purification and Subunit Structure of a High-Molecular-Weight Phosphoprotein Phosphatase (Phosphatase 11) from Rat LiverEuropean Journal of Biochemistry, 1980
- Glycogen synthase phosphatase of rat liver. Its separation from phosphorylase phosphatase on DE-52 columnsBiochemical and Biophysical Research Communications, 1977