Rapid Assay For the Simultaneous Determination of Residues of Oxolinic Acid and Flumequine in Fish Tissues by High-Performance Liquid Chromatography

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Abstract
A simple and rapid method for the simultaneous extraction and determination of residues of oxolinic acid (OX) and flumequine (FQ) in fish tissue, muscle and liver, is presented. the samples were extracted with acetonitrile and ammonia. the drugs were then extracted into a water phase, acidified, and extracted with CHCI3. After evaporation, the sample was redissolved in mobile phase and filtered through a spin-X centrifuge filter. the filtrate was injected onto the HPLC. the calibration curves were linear, and the recovery of oxolinic acid was 101–104 % while the recovery of flumequine was 88–94 %. the detection limits were 5 ng/g for oxolinic acid and 10 ng/g for flumequine.

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