Influence of Oxygen Concentration on the Reduction of Nitrate by a Pseudomonas SP. in the Growing Culture

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Abstract
A method is described whereby O2 tension in growing cultures of micro-organisms may be assessed with reasonable accuracy at any time by means of a recording polarograph. The method applied to a study of nitrate decomposition by a sp. of Pseudomonas, using unadapted cells as an inoculum, reveals that no apparent reduction occurs while O2 is present in soln. A method is also outlined for the rough assessment of substrate deficiency in cultures.