Identification of a novel DRB1-allele (DRB1*0106) by sequence-based typing
- 1 March 1999
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 53 (3) , 308-310
- https://doi.org/10.1034/j.1399-0039.1999.530313.x
Abstract
We report herein the identification of a new DRB1 allele using sequence-based typing. The new allele, DRB1*0106, was detected during routine HLA typing of a patient undergoing bone marrow transplantation. DRB1*0106 is identical to DRB1*0101 except for two codons, 71 (AGG-->GCG) and 86 (GGT-->GTG), changing the encoded arginine to alanine and glycine to valine. Both sequences were confirmed by polymerase chain reaction with sequence-specific primers (PCR-SSP). The polymorphism at codon 71 has not been, until now, identified in DRB1*01 alleles, although it is present in all the DRB1*15 alleles as well as DRB1*1309 and DRB1*1424.Keywords
This publication has 4 references indexed in Scilit:
- HLA class II region sequences, 1998Tissue Antigens, 1998
- Sequencing of HLA class I genes based on the conserved diversity of the noncoding regions: sequencing‐based typing of the HLA‐A geneTissue Antigens, 1997
- HLA‐DR typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor‐recipient matching in cadaveric transplantationTissue Antigens, 1992
- A simple salting out procedure for extracting DNA from human nucleated cellsNucleic Acids Research, 1988