Isolation and Properties of the DNA of African Swine Fever (ASF) Virus

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Abstract
African swine fever (ASF) virus was grown either in swine macrophages or in VERO [African Green monkey kidney] cells and purified free of cell DNA. Virus DNA was isolated from virions as a molecule with a sedimentation coefficient of 60 S and a contour of 58 .+-. 3 .mu.m. These 2 values give a MW of 102 .+-. 5 .times. 106 and 107 .+-. 5 .times. 106, respectively, for the genome of ASF virus. Denatured DNA fragments from ASF virus reassociate with a C0t1/2 [nucleotide concentration .times. incubation time for 50% renaturation] value of 0.60 .+-. 0.05 mol.cntdot.s, which compared with the corresponding value for T4 DNA gives for the molecular mass of ASF virus DNA a value of 102 .+-. 8 .times. 106 daltons. Only virus DNA is synthesized in ASF virus-infected swine macrophages.