Demonstration and solubilization of S-adenosylmethionine: ?-tocopherol methyltransferase from Capsicum chromoplasts

Abstract
Through the use of Capsicum chromoplast membranes, we report for the first time the direct methylation of γ-tocopherol into α-tocopherol in the presence of S-adenosylmethionine. Furthermore the S-adenosylmethionine: γ-tocopherol methyltransferase activity has been solubilized. On a protein basis, the activity recovered in the soluble preparation was higher than that bound to the membranes.

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