Tumor-Specific Immunity in 3-Methylcholanthrene-Induced Murine Fibrosarcomas. I. In Vivo Demonstration of Immunity With Three Preparations of Soluble Antigens 2

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Abstract
The in vivo response to soluble, syngeneic, tumor-specific antigens (TSA) from 3-methylcholanthreneinduced fibrosarcomas was measured by the 3-, 24-, and 48-hour foot pad swelling (FPS) response in mice. Mice immunized to tumor by surgical removal of a growing tumor had specific resistance to challenge with that tumor and specific 3-hour (Arthus) and 24- and 48-hour (delayedhypersensitivity) foot pad responses to preparations containing viable, lyophilized (3M KCI extracts), homogenized, and frozen-thawed extracts, and sonication extracts of tumor cells. Specific FPS was seen in immunized mice with 3M KCI tumor extracts at protein concentrations of 200-10,000 µg/ml. Protein concentrations of 5000 µg/ml appeared to be optimal for testing. Mice given tumor cells had specific 3-hour FPS to KCI-prepared TSA at 7 days after tumor transfer. All responses were negative at 28 days, when tumor diameters were >1.5 cm. Surgical removal of tumor in such mice restored 3−, 24−, and 48hour specific FPSwithin 24 hour

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