Carbon dioxide fixation by reversible pyrrole‐2‐carboxylate decarboxylase from Bacillus megaterium PYR2910

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Abstract
Pyrrole‐2‐carboxylate decarboxylase from Bacillusmegaterium PYR2910 attains a balanced reaction equilibrium with an equilibrium constant of 0.3−0.4 M. Therefore, the enzyme catalyzes the reverse carboxylation of pyrrole after addition of bicarbonate. For the synthesis of pyrrole‐2‐carboxylate, the reverse reaction was optimized and the equilibrium was shifted towards the carboxylate. The product yield was 230 mM (25.5 g/l) pyrrole‐2‐carboxylate from 300 mM pyrrole in a batch reaction and 325 mM (36.1 g/l) from 400 mM pyrrole in a fed‐batch reaction, using both whole cells and the purified enzyme in a pH 8.0 reaction mixture with bicarbonate saturation of 1.9 M. Kinetic studies indicated, that bicarbonate is the reactive species used by this carbon dioxide‐fixation enzyme.

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