Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome
Top Cited Papers
- 31 October 2003
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 302 (5646) , 842-846
- https://doi.org/10.1126/science.1088305
Abstract
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis . Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of ∼30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.Keywords
This publication has 38 references indexed in Scilit:
- Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 2003
- Apparent homology of expressed genes from wood-forming tissues of loblolly pine ( Pinus taeda L.) with Arabidopsis thalianaProceedings of the National Academy of Sciences, 2003
- Regulation of Elongating RNA Polymerase II by Forkhead Transcription Factors in YeastScience, 2003
- Large-Scale Identification of Single-Feature Polymorphisms in Complex GenomesGenome Research, 2003
- Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAsNature, 2002
- A Computer-Based Method of Selecting Clones for a Full-Length cDNA Project: Simultaneous Collection of Negligibly Redundant and Variant cDNAsGenome Research, 2002
- Non–coding RNA genes and the modern RNA worldNature Reviews Genetics, 2001
- Balanced-Size and Long-Size Cloning of Full-Length, Cap-Trapped cDNAs into Vectors of the Novel λ-FLC Family Allows Enhanced Gene Discovery Rate and Functional AnalysisGenomics, 2001
- Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBFNature, 2001
- Genome-Wide Location and Function of DNA Binding ProteinsScience, 2000